LiliF® MG_MS PCR Kit
Background Information
Mycoplasma gallisepticum (MG) is a bacterium belonging to the class Mollicutes and the family Mycoplasmataceae. It is the causative agent of chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys, chickens, game birds, pigeons, and passerine birds of all ages. Mycoplasmosis is the infection of Mycoplasma bacteria. Mycoplasmas have many defining characteristics. Mycoplasma lack cell walls, have highly variable surface proteins and a distinctive plasma membrane, and are the smallest self-replicating prokaryotes. Mycoplasma can cause disease in humans, animals, insects, and plants. Mycoplasma attach to host epithelial cells, such as in the respiratory tract, which causes cell damage and inflammatory response. There are currently over 100 species of Mycoplasma known. The following have been isolated from wild birds: Mycoplasma buteonis, Mycoplasma corogypsi, Mycoplasma falconis, Mycoplasma gypis, Mycoplasma sturni, and Mycoplasma gallisepticum. M. gallisepticum has the most significant effect on wild birds. The bacteria M. synoviae (MS) is a member of the mycoplasma genus. It causes disease in the joints, bones and respiratory system of birds. It is found throughout the world and infection may be referred to as Infectious Synovitis, Avian Mycoplasmosis, Infectious Sinusitis and Mycoplasma Arthritis. It is of economic importance because infection can cause a drop in egg production. The disease is most commonly seen in chickens, and transmission occurs both vertically and horizontally.
LiliF™ MG_MS PCR Kit is able to detect directly and specifically M. gallisepticum and M. synoviae by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of M. gallisepticum and M. synoviae, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.
Principle
• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.
• The assay is a conventional PCR that discriminates M. gallisepticum and M. synoviae in one reaction. The assay is composed of two principal steps: (1) nucleic acid extraction from specimens, and (2) amplification of the target extracted nucleic acid fragment using specific primers pair.
Intended Use
• For Research Use Only, Not for use in diagnostic procedures.
• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.
• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.
- Categories : PCR Kits, Diagnostic Use, Animal Diagnostics
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