Genetics Company For Biotechnology

LiliF® IBV Real-time RT-PCR Kit

Background Information

Avian infectious bronchitis virus (IBV) is a coronavirus which infects chickens, causing the associated disease, infectious bronchitis (IB). It is a highly infectious avian pathogen which affects the respiratory tract, gut, kidney and reproductive systems of chickens. There is also evidence that IBV can infect other avian species. IBV affects the performance of both meat producing and egg producing chickens and is responsible for substantial economic loss within the poultry industry. IBV is classified a coronavirus, in the genus Gammacoronavirus, or group 3, with a non-segmented, positive-sense single-stranded RNA genome. When inhaled, virus will attach to glycoprotein receptors containing sialic acid on ciliated epithelial cells of the respiratory epithelium.

The respiratory replication will result in loss of ciliary activity, mucus accumulation, necrosis and desquamation, causing respiratory distress, râles and asphyxia. Local virus replication will result in viremia, spreading the infection into other tissues and organs. Other respiratory diseases of chickens (Mycoplasma gallisepticum, avian infectious laryngotracheitis (Gallid herpesvirus 1), Newcastle disease (avian paramyxovirus 1), avian metapneumovirus infection may be confused clinically to infectious bronchitis. Through viremia, some nephrotropic strains (most of high virulence) could infect the kidney epithelium in tubules and nephron, causing kidney failure. At gross examination, kidneys may appear swollen and pale in color and with urates in ureters. In hens, the viremic IBV will also reach the oviduct, causing lesions in the magnum (the egg-white gland) and in the uterus (the egg-shell gland), leading to a sharp decline of egg production, shell-less, fragile or roughened shells eggs (uterus lesion) with watery whites (magnum lesion). Infection of chickens at puberty, during the oviduct development, will impede oviduct formation and destroy future laying capacity, resulting in "false layers". However, other diseases affecting layer chickens could lead to that condition.

LiliF™ IBV Real-time RT-PCR Kit is able to detect directly and specifically IBV by real-time PCR technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer and probe set for a highly conserved region based on current sequence alignments of all type of IBV allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the real-time RT-PCR (quantitative) method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative Real-time PCR testing product with 5’ nuclease assay technology and CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• The assay is a real-time RT-PCR that discriminates IBV in one reaction. The assay is composed of two principal steps: (1) nucleic acid extraction from specimens, and (2) amplification of the target extracted nucleic acid fragment using fluorescent probe and specific primers pair.

• The internal positive control (IPC) has been introduced to individual tube of the kit to verify the successful amplification reaction. The IPC is co-amplified with target band from clinical specimens.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.