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Trypsin 0,25%-EDTA in HBSS w/o Calcium w/o Magnesium w/ Phenol Red
- Size : 100 ml
Trypsin 0.25%-EDTA in Hank's Balanced Salt Solution (HBSS) without Calcium and Magnesium with Phenol Red is a specialized enzymatic solution used in cell culture applications for the detachment and dissociation of adherent cells from the culture vessel surface. This solution contains trypsin, a protease enzyme, combined with ethylenediaminetetraacetic acid (EDTA), which acts as a chelating agent to inhibit the activity of trypsin and prevent cell clumping.
The Hank's Balanced Salt Solution (HBSS) serves as a balanced electrolyte solution that helps maintain the osmotic balance and pH of the cell culture environment. It is formulated without calcium and magnesium ions to prevent interference with trypsin activity and maintain cell viability during the detachment process.
The addition of Phenol Red to the solution allows for visual monitoring of pH changes in the medium, as it acts as a pH indicator, turning from red to yellow as the pH decreases. This provides a convenient way to assess the readiness of the trypsinization process.
Trypsin-EDTA in HBSS without Calcium and Magnesium with Phenol Red is commonly used in routine cell culture procedures for the dissociation of various adherent cell types, including mammalian cell lines and primary cells, prior to subculture or experimental assays. It offers convenience, reliability, and consistent performance in cell detachment applications.
- Categories : Cell Culture, Media,
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