Genetics Company For Biotechnology

Trypsin 2.5% in HBSS w/o Calcium w/o Magnesium w/o Phenol Red, w Sodium Bicarbonate

Trypsin 2.5% in HBSS (Hank's Balanced Salt Solution) without Calcium, Magnesium, and Phenol Red, with Sodium Bicarbonate is a commonly used enzyme solution in cell culture applications. It contains trypsin, a proteolytic enzyme that helps detach adherent cells from culture vessels by cleaving cell surface proteins. This formulation of trypsin is specifically designed to maintain a stable pH and osmolarity, which is essential for cell viability during detachment and subsequent subculture. The absence of calcium and magnesium ions prevents interference with the trypsin activity, while the addition of sodium bicarbonate helps maintain physiological pH levels. This trypsin solution is ideal for routine cell passaging and dissociation in cell culture workflows.