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Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red
Trypsin-EDTA 1X in Phosphate Buffered Saline (PBS) without Calcium and Magnesium and without Phenol Red is a specialized enzyme solution used in cell culture applications for the detachment and dissociation of adherent cells from the culture vessel surface.
The solution contains trypsin, a protease enzyme, combined with ethylenediaminetetraacetic acid (EDTA), which acts as a chelating agent to inhibit the activity of trypsin and prevent cell clumping.
The PBS serves as a balanced electrolyte solution that helps maintain the osmotic balance and pH of the cell culture environment. It is formulated without calcium and magnesium ions to prevent interference with trypsin activity and maintain cell viability during the detachment process.
The absence of Phenol Red in the solution eliminates the need for visual monitoring of pH changes during trypsinization, making it suitable for applications where Phenol Red interference is undesirable.
Trypsin-EDTA in PBS without Calcium and Magnesium and without Phenol Red is commonly used in cell culture procedures for the dissociation of various adherent cell types, including mammalian cell lines and primary cells, prior to subculture or experimental assays. It offers convenience, reliability, and consistent performance in cell detachment applications.
- Categories : Cell Culture, Media,
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