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Colcemid 10 μg/ml in PBS
- Size : 10 μg/ml
Colcemid 10 μg/ml in PBS is a specialized solution used in cell biology and cytogenetics research for the synchronization of cells in mitotic phase. Colcemid, a derivative of the natural compound colchicine, acts by disrupting the assembly of microtubules, thereby arresting cells in metaphase of mitosis.
This solution is typically prepared at a concentration of 10 μg/ml in phosphate-buffered saline (PBS), ensuring stability and compatibility with various cell culture applications. Colcemid treatment induces cell cycle arrest at metaphase, facilitating the collection of synchronized cells for downstream experiments such as karyotyping, chromosome analysis, and cell cycle studies.
The use of Colcemid 10 μg/ml in PBS allows researchers to obtain a uniform population of cells arrested at a specific stage of mitosis, enabling accurate and reproducible results in cytogenetic analyses. Its standardized formulation and reliability make it an essential tool for investigating chromosomal abnormalities, cell division dynamics, and other cellular processes.
- Categories : Cell Culture, Media,
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