DNBSEQ-G99 Sequencers
As one of the sequencers with the fastest speed among mid-low throughput in the world, DNBSEQ-G99 is developed based on MGI's core DNBSEQTM sequencing technology, enabled by innovations in biochemistry, optics, fluidics, temperature control, and other core systems. DNBSEQ-G99 is especially applicable for multiple sequencing methods, it only needs 12 hours to finish PE150 sequencing.
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8 Gb~96 Gb per ru |
Only 12 hrs for PE150 (from loading to FASTQ)
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Supports multiple read lengths, including PE50, SE100, PE150, PE300, SE400 |
Applicable to multiple sequencing methods |
Performance Parameter
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Maximum number of Flow cells |
Lanes/Flow cell |
Effective Reads*/Flow Cell |
Supported Reads Lengths*** | Data Output | Q30** |
Run Time |
|---|---|---|---|---|---|---|
|
2 |
1 |
80 M |
SE100/PE50 |
8 Gb~16 Gb | ＞90% | 5 h |
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PE150 |
24 Gb~48 Gb |
＞85% | 12 h | |||
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PE300 |
48 Gb~96 Gb |
＞85% | 30 h | |||
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SE400 |
32 Gb~64 Gb |
＞75% | 20 h | |||
| * The effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method. | ||||||
| ** The percentage of bases above Q30 is the average of an internal standard library over the entire run. Actual performance is affected by factors such as sample type, library quality, and insert fragment length. | ||||||
| *** DNBSEQ-G99 also supports SE50 and PE100 sequencing, and the existing kits can support SE50, PE100 read length sequencing. | ||||||
| Method | Application |
Recommended read length |
Data size | Sample per Run | |
|---|---|---|---|---|---|
|
1 flow cell |
2 flow cells | ||||
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Targeted Capture/ Multiplex PCR |
Oncology panel |
PE100/ PE150 |
Small panel: ~1 Gb/Sample |
24 | 48 |
| Genetic disease diagnosis (small panel) | PE150 |
Deafiness: ~5 Gb/Sample Thalassemia: ~0.2 M reads/Sample |
4 400 |
8 800 |
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|
ATOPlex panel |
PE100/ PE150 |
Respiratory tract panel: 5 M reads/Sample SARS-CoV-2 panel: 5 M reads/Sample |
16 | 32 | |
| WES | PE150 | 15 Gb/Sample | 1~2 | 2~4 | |
| Methylation | Oncology targeted methylation panel | PE150 | ~5 Gb/Sample | 4 | 8 |
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Small Genome Sequencing |
Metagenomics for pathogen detection | SE50/ SE100 | Meta: 20 M reads/Sampple | 4 | 8 |
| Microbial WGS | PE100/ PE150 | Single bacterium: ~1 Gb/Sample | 16~24 | 32~48 | |
| 16S Metagenomic Sequencing | PE300 | ≥0.1 M reads /Sample | 576 | 1152 | |
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LOW pass whole-genome sequencing |
NIPT | SE50 |
~10 M reads/Sample |
8 |
16 |
| PGS | SE50 | ||||
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Transcriptome sequencing
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RNA-Seq | SE50/ PE150 |
Expression profiling: ~25 M reads/Sample Transcriptome: ~6 Gb/Sample |
3 4 |
6 8 |
| Frensics | DNA signature identification | SE400 | ~0.8 M reads/sample | 96 | 192 |
| *Methods indicated with are the key applications of DNBSEQ-G99. | |||||
| ** Recommended data output and sample numbers are only for reference, actual application will require optimisation adjustments. | |||||
- Categories : Instrument, Molecular Instruments, NGS
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