i-Taqâ„¢ DNA Polymerase 250 unit
Description
High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions
• 94 KDa thermostable DNA polymerase
• High purity Taq DNA Polymerase - Removal of E. coli -derived proteins and DNA that may act as PCR sources
• Applicable to DNA from cloned DNA to human genomic DNA
• Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions i-Taq ™ DNA Polymerase is a thermostable DNA polymerase of 94 kDa, which is expressed in E. coli by cloning the polymerase gene of Thermus aquaticus (strain YT1).
It removes the E. coli-derived protein and DNA which can act as a contaminant in PCR, It is stable and efficient DNA amplification product. Genomic DNA, cDNA, etc. can be amplified up to 5 Kb. PCR (Polymerase Chain Reaction) is a method of amplifying a desired DNA molecule in a test tube by specifically repeating synthesis of a specific DNA site. As a result, it is possible to synthesize a large amount of DNA using a very small amount of DNA, Of course, it is widely used in the fields of basic biology, medicine, and biology. We are now expanding the scope of its use in the fields of forensic medicine, food, environmental hygiene, and animal and plant inspection in a simple and quick way.
DNA polymerase chain reaction can amplify DNA of specific site in a few hours up to 105-108 times in vitro. The amplified DNA can be used for various experiments as follows. Based on the experimental results, various medical researches Can be applied to. In the early days when this method was developed, PCR was performed using Escherichia coli DNA Polymers, so it was necessary to supplement DNA polymerase of denatured Escherichia coli at every denaturation. However, by using the thermostable DNA Polymerase isolated from Thermus aquaticus in PCR, we did not have to do the troublesome task of supplementing the enzyme every step. As a result, PCR became an indispensable method for molecular biology. PCR has a series of three steps and repeats 30 to 40 times.
- Categories : PCR Kits, Research Applications, Conventional PCR
