One Step TB Green™ PrimeScript™ RT-PCR Kit II (Perfect Real Time)
 
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One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time)

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RR066A One-Step TB Green® PrimeScript™ RT-PCR Kit (Perfect Real Time) 100 Rxns


The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) is a quantitative real-time PCR kit designed for intercalating dye-enabled product detection. Using this TB Green Real-Time PCR (qPCR) kit, RT-PCR can be performed in a single tube, minimizing pipetting steps and contamination risk. Reaction products are monitored in real time, eliminating the need for electrophoresis verification following PCR. The sensitivity of the enzyme offered in this intercalator real-time PCR kit enables detection of very small amounts of RNA, including RNA from viral, rare, and precious samples.


Overview

  • Quantitative real-time PCR enables rapid and accurate analysis of RNA from viral, rare, or precious samples.
  • Takara Ex Taq HS is a high-efficiency hot-start PCR enzyme that can be used in RT-PCR.
  • The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) buffer system is optimized for quantitative real-time PCR, allowing efficient amplification and highly sensitive detection.
  • One-Step TB Green RT-PCR Buffer IV is a 2X TB Green premix; the premix formulation simplifies reaction mixture preparation.

More Information

Applications

  • Quantitative real-time PCR
  • Intercalating dye-based real-time PCR (qPCR)
  • Detection and analysis of small RNA amounts

Compatible real-time PCR instruments

  • Thermal Cycler Dice Real Time System
  • ABI PRISM 7000/7700, Applied Biosystems 7300/7500 Real-Time PCR System (Applied Biosystems)
  • LightCycler (Roche Diagnostics)

Product citations

Arita, M., Wakita, T. & Shimizu, H. Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity. J. Gen. Virol. 89, 2518-30 (2008).

Murata, T. et al. Involvement of Jun dimerization protein 2 (JDP2) in the maintenance of Epstein-Barr virus latency. J. Biol. Chem. 286, 22007-16 (2011).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
 
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