Description
RT-PCR PreMix kit of master mix type for One-step RT-PCR (cDNA synthesis and PCR at once) by using gene specificprimer.
· One-step RT-PCR
· Ready to Use
All components required for RT-PCR in one tube
Easy use by adding RNA, gene specific primer and DW only
· Sensitive cDNA synthesis and highly specific PCR amplification
· Adjustable reaction volume according to requirement
· High specificity and accuracy by hot-start Taq DNA Polymerase
RT-PCR PreMix kit is master mix type product for One-step RT-PCR (cDNA synthesis and PCR at once) by using gene specific primer.
For first-strand cDNA synthesis from total RNA and mRNA template and the chain reaction in a tube using GSP (Gene Specific Primer), the product includes reverse transcriptase, DNA polymerase and all components for each reaction are mixed in a solution.
OptiScript™ RT System applied to ONE-STEP RT-PCR PreMix Kit helps the accurate and highly effective reverse transcription from RNA template in 1 pg up to 2 ug. This is also designed to synthesize the first cDNA and perform high specific PCR. The gene expression in low copy can work with the product which can be stable for a year under the freezing condition as it was designed not to be frozen at freezer.
● Applications
· RNA qualification study
· RNA expression study
· Specific RNA detection
· Virus research
· Molecular diagnostic study
· Real-time RT-PCR, etc.
