PrimeScriptâ„¢ RT Master Mix (Perfect Real Time)
 
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Real-time RT-PCR Kit: PrimeScript RT Master Mix (Perfect Real Time)

To minimize sample handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble - just add RNA template and water. The kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase Inhibitor, random 6mers, Oligo dT Primer, dNTP Mixture, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. This kit is designed for two-step real-time RT-PCR. Use it upstream of a SYBR or TaqMan qPCR premix from Takara Bio, such as SYBR Premix Ex Taq II (Tli RNase H)SYBR Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).


Features

  • Reverse transcription in just 15 minutes
  • Master Mix format reduces pipetting steps and the possibility of contamination
  • Works efficiently with GC-rich and.or higher-structured RNA templates
  • Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
 
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