AMV Reverse Transcriptase XL for RT-PCR
 
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AMV Reverse Transcriptase XL synthesizes complementary full-length cDNA from single-stranded RNA, DNA or RNA-DNA hybrid template. The enzyme requires a primer to initiate synthesis and Mg2+or Mn2+ for activity. AMV Reverse Transcriptase XL is positive for 5'->3' ribonuclease H activity and can be used for a number of applications including reverse transcription.

 

AMV Reverse Transcriptase XL is highly purified, free of extraneous nucleases and is qualified for use in full-length cDNA synthesis and reverse transcription using RNA templates that are up to 10 kb in length. It can be used over a temperature range of 37–55°C but maximal yield of full-length cDNA product is achieved between 41–44°C.


Applications

  • Reverse transcription
  • Full-length cDNA synthesis
  • Primer extension
  • Preparation of DNA probes
 
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