Clontech Easy Yeast Plasmid Isolation Kit
 
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The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.

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The Easy Yeast Plasmid Isolation Kit provides a simple and efficient plasmid mini-prep method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed intoE. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.

Features

  • Rescue plasmids from yeast using spin columns
  • Highly efficient Zymolyase is included
  • Purify DNA in less time

Applications

  • Plasmid DNA mini-preps from yeast
  • Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen
 
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