Spin type product with 2 column which can extract total RNA from plant
such as various leaf, trunk, root and seed.
· Extraction of total RNA from various plant sample
Effective
extraction of total RNA from seed containing a lot of metabolites
· Extraction of high purity and yield RNA
The
fast removal of gDNA and extraction of RNA within 20 minutes by 2 column system
The
removal of more than 99% of gDNA without extra DNase treatment
· RNA extraction based on non-phenol method
IQeasy™ Plus Plant RNA Extraction Mini Kit is designed to
extract total RNA from leaf, trunk, root and seed of various plants.
Plant accumulates variety of metabolites through
photosynthesis and those metabolites would be an inhibitor which has an effect on extraction of nucleic acid.
To remove this inhibitor, high concentration chaotrophic salt or phenol should be used under lysis step. However, for rice,
barley, wheat and seed containing a lot of starch or bean, green gram, pea and
seed containing a lot of protein as well as soil, if alcohol is added for
binding, too much precipitation occurs and also the nucleic acid is precipitated
(co-precipitation), that brings the sample including less RNA. To overcome this
problem, IQeasy™ Plus Plant RNA Extraction Mini Kit was developed.
IQeasy™ Plus Plant RNA Extraction Mini Kit works effectively on the plant
containing a lot of metabolites for extraction of total RNA and the high yield and purity RNA can
be extracted because chaotrophic salt is not used and inhibitor, the factor
of obstruction is removed under lysis step.
2 column system is used, one is a sieve spin column which
can remove the supernatant fast in homogenate and another is a gDNA binding
column for removal of gDNA.
As mentioned earlier, the extraction of RNA from various
plant can be done within 20 minutes because of the absence of enzymatic lysis. Two kinds
of protocol are provided for both tissue and species for easy
and simple extraction of high purity and yield total RNA.
IQeasy™ plus Series Total RNA Extraction Mini Kit is a
special product for RNA extraction which effectively extracts total RNA from
various samples(Table 1). According to the characteristics of samples, the
specialized tool is provided , e.g. lysis buffer, a removal of inhibitor and a
minimum of gDNA mix, etc.
This is designed
and developed to extract total RNA of high yield and purity and raise the
reproducibility through the
specialized protocol for each sample.
CAPS (Clean Automatic Packaging System) is applied to all
IQeasy™ plus Series Kit in order to minimize contamination and it can make
reliable pathogen research available(table 2). RNA extracted by IQeasy™
plus Series can be used for not only pathogen research but also microarray.
